Translational_Unit

Part:BBa_K4951070

Designed by: Julia Vu   Group: iGEM23_Stanford   (2023-10-11)


pTwist Kan High Copy mScarlet3 - Insert (Trigger)

The pTwist mScarlet3 plasmid, specifically the KanR gene, was intended to serve as the trigger sequence that our toehold switches were designed against for in vivo toehold switch testing.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 23


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